PB2801_EE

Cbrenneri Dev Timecourse Expression PB2801_EE RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. brenneri. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.

Protocols

  1. Growth and isolation: Worm_growth, Staging_and_isolation, RNA_isolation, polyA Selection, Library preparation for Illumina Sequencing
  2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina paired-end sequencing on GAIIx, Illumina paired-end sequencing on HiSeq2000
  3. Other Protocols: Alignment, Normalization
  1. External Links: SRR352227, SRR352228, SRR352229


Release Date: 2012-09-27