2.5% EtOH L3

Dm Treatment Expression RNA-seq 2.5% EtOH Third Instar larvae uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Reads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster 2.5% EtOH Third Instar larvae were aligned to the D. melanogaster r5 genome using Bowtie and to predicted splice junctions using SPA, retaining only uniquely mapped reads. Tracks show read alignments and density.
Total read count: 104693048
Mapped read count: 96879760
Alignment rate: 92%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: SRR103730, SRR103731

Related modENCODE submissions:

• modENCODE_4271: Dm_Treatment_Expression_RNA-seq_2.5%_EtOH_Third_Instar_larvae_sequencing

Release Date: 2012-02-07