Cd 0.05mM CdCl2 Late L3 12hr

Dm Treatment Expression RNA-seq Cd Treatment_0.05mM CdCl2_Late Third Instar Larvae_12hr uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Reads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster Cd Treatment 0.05mM CdCl2 Late Third Instar Larvae 12hr were aligned to the D. melanogaster r5 genome using Bowtie and to predicted splice junctions using SPA, retaining only uniquely mapped reads. Tracks show read alignments and density.
Total read count: 169610044
Mapped read count: 93271687
Alignment rate: 54%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: SRR103736, SRR103737

Related modENCODE submissions:

• modENCODE_4277: Dm_Treatment_Expression_RNA-seq_Cd_Treatment_0.05mM_CdCl2_Late_Third_Instar_Larvae_12hr_sequencing

Release Date: 2012-02-07