Heat shock 1hr 36C, wet heat 30min 25C Adults 4day

Dm Treatment Expression RNA-seq Heat shock 1hr@36C wet heat 30min@25C_Four Day Adults uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Reads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster Heat shock 1hr@36C wet heat 30min@25C Four Day Adults were aligned to the D. melanogaster r5 genome using Bowtie and to predicted splice junctions using SPA, retaining only uniquely mapped reads. Tracks show read alignments and density.
Total read count: 124896854
Mapped read count: 113834388
Alignment rate: 91%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: SRR103723, SRR103724

Related modENCODE submissions:

• modENCODE_4284: Dm_Treatment_Expression_RNA-seq_Heat_shock_1hr@36C_wet_heat_30min@25C_Four_Day_Adults_sequences

Release Date: 2012-02-07