Heat shock 1hr 36C, wet heat 30min 25C Adults 4dayDm Treatment Expression RNA-seq Heat shock 1hr@36C wet heat 30min@25C_Four Day Adults uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)DetailsReads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster Heat shock 1hr@36C wet heat 30min@25C Four Day Adults were aligned to the D. melanogaster r5 genome using Bowtie and to predicted splice junctions using SPA, retaining only uniquely mapped reads. Tracks show read alignments and density. General DescriptionRNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011) Protocols
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Release Date: 2012-02-07 |