Paraquat 10mM Adults 4day

Dm Treatment Expression RNA-seq Paraquat Treatment_10mM_Four Day Adults uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Reads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster Paraquat Treatment 10mM Four Day Adults were aligned to the D. melanogaster r5 genome using Bowtie and to predicted splice junctions using SPA, retaining only uniquely mapped reads. Tracks show read alignments and density.
Total read count: 121504562
Mapped read count: 112816868
Alignment rate: 92%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: SRR103721, SRR103722

Related modENCODE submissions:

• modENCODE_4285: Dm_Treatment_Expression_RNA-seq_Paraquat_Treatment_10mM_Four_Day_Adults_sequencing

Release Date: 2012-02-07