Adult Mated Female_Ecl+1day Heads, Multi mapper

Dm Tissue Expression RNA-seq mated female eclosion + 1 day heads multiply mapping reads (Celniker project, Graveley subgroup)

Details

Stranded RNA-seq analysis was performed in biological replicate on poly(A)+ RNA extracted from mated female heads of D. melanogaster, 1 day after eclosion. Uniquely aligned reads were mapped using Bowtie. Tracks show alignments and read density.

General Description

Stranded RNA-seq analysis was performed on poly(A)+ RNA from various tissues dissected from various stages of D. melanogaster. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and strand-specific library construction were performed in the Brenton Graveley lab. Libraries were distributed among 3 labs in the Drosophila Transcriptome group for sequencing (Celniker, Gingeras, and Graveley) for paired-end RNA sequencing (2x76+ nt) on the GAIIx platform. Fastq files were generated using pipeline version 1.5. Reads were aligned to the genome using TopHat.

Protocols

  1. Sample preparation: RNA library prep
  2. Other Protocols: Celniker paired_end sequencing, TopHat stranded RNAseq alignment, Graveley paired_end sequencing

Related modENCODE submissions:


Release Date: 2011-06-20