Cell Lines

Cell Lines: TotalRNA Signal intensities (Celniker project, Gingeras subgroup)

General Description

We will generate over 600 RNA samples in biological triplicate and use them to generate expression profile maps detailing the sites of transcription across the fly genome using whole genome tiling arrays at 38 bp resolution as a broad survey of the transcriptome and 7 bp arrays resolution to identify at high resolution transcripts ends, splice sites, and small RNAs.

Protocols

  1. Growth and isolation: RNA_extraction
  2. Sample preparation: CDNA_synthesis_for_Expression_Arrays, Labeling_of_cDNA_for_Expression_Arrays, Array_Hybridization_for_Expression_Arrays, Array_Scanning_for_Expression_Arrays
  3. Data Analysis: Data_Normalization_for_Expression_Arrays, Signal Graph generation, Transfrag Analysis

Reagents

  1. Animals:
    mbn2, Drosophila melanogaster CME W2, Drosophila melanogaster 1182-4H, Drosophila melanogaster CME L1, Drosophila melanogaster CME W1 Cl.8+, Drosophila melanogaster GM2, Drosophila melanogaster Kc167, Drosophila melanogaster ML-DmBG1-c1, Drosophila melanogaster ML-DmBG3-c2, Drosophila melanogaster ML-DmD11, Drosophila melanogaster ML-DmD17-c3, Drosophila melanogaster ML-DmD20-c2, Drosophila melanogaster ML-DmD20-c5, Drosophila melanogaster ML-DmD21, Drosophila melanogaster ML-DmD32, Drosophila melanogaster ML-DmD4-c1, Drosophila melanogaster ML-DmD8, Drosophila melanogaster ML-DmD9, Drosophila melanogaster S1, Drosophila melanogaster S2-DRSC, Drosophila melanogaster S2R+, Drosophila melanogaster S3, Drosophila melanogaster Sg4, Drosophila melanogaster ML-DmD16-c3, Drosophila melanogaster
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R

Release Date: 2009-01-27