Caffeine 1.5 mg/ml L3

Dm Treatment Expression RNA-seq Caffeine Treatment_1.5 mg/ml_Third instar larvae multiply mapping reads (Celniker project, Celniker subgroup)

Details

Reads sequenced from stranded RNA libraries made from Third Instar Larvae Oregon-R Drosophila melanogaster treated with Caffeine Treatment 1.5 mg ml Third instar larvae were aligned to the D. melanogaster r5 genome using TopHat (allowing multiple genomic alignments with up to two mismatches).
Total read count: 158283264
Mapped read count: 144985593
Alignment rate: 91%

General Description

Stranded RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster genome using TopHat.

Protocols

  1. Data Analysis: TopHat stranded RNAseq alignment
  1. External Links: SRR103732, SRR103733

Related modENCODE submissions:


Release Date: 2012-02-07