Cold Method 2 Adults 4day

Dm Treatment Expression RNA-seq Cold Treatment_Method 2_Four Day Adults multiply mapping reads (Celniker project, Celniker subgroup)

Details

Reads sequenced from stranded RNA libraries made from Four Day Adults Oregon-R Drosophila melanogaster treated with Cold Treatment Method 2 were aligned to the D. melanogaster r5 genome using TopHat (allowing multiple genomic alignments with up to two mismatches).
Total read count: 117126310
Mapped read count: 106477108
Alignment rate: 90%

General Description

Stranded RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster genome using TopHat.

Protocols

  1. Data Analysis: TopHat stranded RNAseq alignment
  1. External Links: SRR103710, SRR103711

Related modENCODE submissions:


Release Date: 2012-02-07