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Embryo 0-4h RNA-seq ( project, White subgroup)
RNA-seq was performed on polyA-RNA for developmental stages of Drosophila melanogaster on which ChIP-chip or ChIP-seq has been performed. The RNA was isolated from the same material used for ChIP experiments and, after purification and production of the Solexa libraries, sequenced (single-end) at Argonne National Lab. For RNA-seq, 2 lanes per sample are bieng run.
- Growth and isolation: Animal Staging & collection, MRNA Extraction for Illumina Sequencing, RNA-seq Library Preparation for Illumina Sequencing
- Sample preparation: RNA-seq Library Preparation for Illumina Sequencing, Solexa_RNAseq
- Data Analysis: TopHat RNA-seq Alignments:KW:1
- Animals/Lines: Embryo 0-4h, Embryo_0-4h_TotalRNA, y; Gr22b Gr22d cn CG33964[R4.2] bw sp; LysC MstProx GstD5 Rh6