Embryo 2-4h RNA-Seq Unique mapper

Dm Developmental Timecourse Expression RNA-seq em2-4hr uniquely mapping reads (Celniker project, Graveley subgroup)

Details

Unstranded libraries were created from polyA+ RNA isolated from staged em2-4hr y cn bw sp D. melanogaster flies, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 130345792
Mapped read count: 62867057
Alignment rate: 48%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Unstranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

  1. Data Analysis: Stranded paired-end alignment
  1. External Links: GEO:TMPID:SRR023502, GEO:TMPID:SRR023660, GEO:TMPID:SRR023705, GEO:TMPID:SRR023722, GEO:TMPID:SRR023745, GEO:TMPID:SRR027112, SRR023502, SRR023660, SRR023705, SRR023722, SRR023745, SRR027112

Related modENCODE submissions:


Release Date: 2012-03-20