Embryo 14-16h RNA-Seq Unique mapper

Dm Developmental Timecourse Expression RNA-seq em14-16hr uniquely mapping reads (Celniker project, Graveley subgroup)

Details

Unstranded libraries were created from polyA+ RNA isolated from staged em14-16hr y cn bw sp D. melanogaster flies, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 262382683
Mapped read count: 72245981
Alignment rate: 27%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Unstranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

  1. Data Analysis: Stranded paired-end alignment
  1. External Links: GEO:TMPID:SRR023540, GEO:TMPID:SRR023641, GEO:TMPID:SRR023725, GEO:TMPID:SRR023734, GEO:TMPID:SRR023736, GEO:TMPID:SRR023822, GEO:TMPID:SRR024011, GEO:TMPID:SRR035400, GEO:TMPID:SRR035401, SRR023540, SRR023641, SRR023725, SRR023734, SRR023736, SRR023822, SRR024011, SRR035400, SRR035401

Related modENCODE submissions:


Release Date: 2012-03-20