Embryonic (7b array, poly A)

Dm_y[1]cn[1] bw[1] sp[1]_embryo_4-6h_Poly-Ap_p200_409-410-411_7bp (Celniker project, Gingeras subgroup)

Details

RNA was extracted from Drosophila y cn bw sp 4-6h embryos, and applied to a 7bp tiling array to assay for transcriptional activity. Tracks show signal intensity and derived transcriptionally active regions.

General Description

We will generate over 600 RNA samples in biological triplicate and use them to generate expression profile maps detailing the sites of transcription across the fly genome using whole genome tiling arrays at 38 bp resolution as a broad survey of the transcriptome and 7 bp arrays resolution to identify at high resolution transcripts ends, splice sites, and small RNAs.

Protocols

  1. Sample preparation: Labeling_of_cDNA_for_Expression_Arrays, Array_Scanning_for_Expression_Arrays, Array_Hybridization_for_Expression_Arrays, CDNA_synthesis_for_Expression_Arrays
  2. Data Analysis: Transfrag Analysis, Data_Normalization_for_Expression_Arrays, Signal Graph generation

Experimental Reagents

  1. Arrays: Drosophila tiling array C03 7bp, Drosophila tiling array C01 7bp, Drosophila tiling array C02 7bp, Drosophila tiling array C05 7bp, Drosophila tiling array C04 7bp

Sample Details

    Animals/Lines:
  1. Samples: Celniker/RNA:409, Celniker/RNA:411, Celniker/RNA:410

Related modENCODE submissions:


Release Date: 2010-10-29