Embryonic (PolyA)

Embryonic Signal Intensities (Celniker project, Gingeras subgroup)

General Description

We will generate over 600 RNA samples in biological triplicate and use them to generate expression profile maps detailing the sites of transcription across the fly genome using whole genome tiling arrays at 38 bp resolution as a broad survey of the transcriptome and 7 bp arrays resolution to identify at high resolution transcripts ends, splice sites, and small RNAs.

Protocols

  1. Growth and isolation: Population Cages, Embryo Collection, RNA_extraction
  2. Sample preparation: CDNA_synthesis_for_Expression_Arrays, Labeling_of_cDNA_for_Expression_Arrays, Array_Hybridization_for_Expression_Arrays, Array_Scanning_for_Expression_Arrays
  3. Data Analysis: Data_Normalization_for_Expression_Arrays, Signal Graph generation, Transfrag Analysis

Reagents

  1. Animals: Embryo 0-2h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 2-4 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 4-6 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 8-10 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 10-12 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 12-14 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 14-16 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 16-18 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 18-20 h, Drosophila melanogaster, Y_cn_bw_sp, Embyro 22-24 h, Drosophila melanogaster, Y_cn_bw_sp
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R
  3. Samples: Celniker/RNA:403, Celniker/RNA:404, Celniker/RNA:405, Celniker/RNA:415, Celniker/RNA:416, Celniker/RNA:417, Celniker/RNA:418, Celniker/RNA:419, Celniker/RNA:420, Celniker/RNA:421, Celniker/RNA:422, Celniker/RNA:423, Celniker/RNA:427, Celniker/RNA:428, Celniker/RNA:429, Celniker/RNA:433, Celniker/RNA:434, Celniker/RNA:435, Celniker/RNA:406, Celniker/RNA:407, Celniker/RNA:408, Celniker/RNA:409, Celniker/RNA:410, Celniker/RNA:411, Celniker/RNA:412, Celniker/RNA:413, Celniker/RNA:414
Release Date: 2009-01-27