GM2, Unique mapperD.melanogaster Cell Line Expression RNA-seq GM2 uniquely mapping reads (Celniker project, Graveley subgroup)DetailsStranded libraries were created from polyA+ RNA isolated from the D. melanogaster cell line GM2, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density. General DescriptionRNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Reads were aligned to unique locations in the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011). Protocols
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Release Date: 2012-03-19 |