GM2, Unique mapper

D.melanogaster Cell Line Expression RNA-seq GM2 uniquely mapping reads (Celniker project, Graveley subgroup)

Details

Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster cell line GM2, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 63728283
Mapped read count: 40426360
Alignment rate: 63%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Reads were aligned to unique locations in the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011).

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: GEO:TMPID:SRR070265, GEO:TMPID:SRR070278, SRR070265, SRR070278

Related modENCODE submissions:

• modENCODE_4723: Dm Cell Line Expression stranded RNA-seq GM2 sequences

Release Date: 2012-03-19