Drosophila Variant Histone H3.3 Binding Sites (Henikoff Lab). Chromatin was prepared from Drosophila S2 cells engineered to express a histone variant, H3.3, that was tagged in vivo with biotin. After digestion with micrococcal nuclease and collection with streptavidin-coated beads, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down by the streptavidin beads. Scaled log2(ratios) are shown in this track. [PMID: 17347439]