Fly Mononucleosomes (Henikoff project, Henikoff subgroup)

General Description

Chromatin from Drosophila was digested with micrococcal nuclease and extracted with 15 mM NaCl and sheared, followed by gel electrophoresis. The gel-purified, mononucleosome length DNA fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.


  1. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  2. Other Protocols: Nuclei Chromatin Extraction, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: S2-Mt-Bio-H2Av, S2-Mt-Bio-H3.3
  2. External Links: GSM333841, GSM333837, GSM333840, GSM333844, GSM333835

Release Date: 2009-03-22 Nucleosome Density Release Date: 2009-05-03 Mononucleosomes H2Av Release Date: 2009-11-30 Mononucleosomes H3.3