Mononucleosomes

Fly Mononucleosomes (Henikoff project, Henikoff subgroup)

General Description

Chromatin from Drosophila was digested with micrococcal nuclease and extracted with 15 mM NaCl and sheared, followed by gel electrophoresis. The gel-purified, mononucleosome length DNA fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.

Protocols

  1. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  2. Other Protocols: Nuclei Chromatin Extraction, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: S2-Mt-Bio-H2Av, S2-Mt-Bio-H3.3
  2. External Links: GSM333841, GSM333837, GSM333840, GSM333844, GSM333835

Release Date: 2009-03-22 Nucleosome Density Release Date: 2009-05-03 Mononucleosomes H2Av Release Date: 2009-11-30 Mononucleosomes H3.3