Chromatin NaCl fractions (embryonic samples)

Fly embryos Salt Extracted Chromatin (Henikoff project,Henikoff subgroup)

General Description

Chromatin from Drosophila embryos, staged 4 - 6 hr. after egg-laying, was digested with micrococcal nuclease and extracted with 600 mM NaCl. The pellet fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.

Protocols

  1. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  2. Other Protocols: NimbleGen Scaling, Nuclei Chromatin Extraction

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: B3


Release Date: 2008-12-16