Chromatin NaCl fractions (Histone Variants)

Salt Extracted Chromatin from culture cells with biotin-tagged Histone Variants (Henikoff project,Henikoff subgroup)

General Description

Chromatin from Drosophila S2 tissue culture cells engineered to express biotin-tagged histone H2Av (note that the transgene was not induced during this particular experiment) was digested with micrococcal nuclease and extracted with 150 mM NaCl, followed by 600 mM NaCl. The soluble fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.

Protocols

  1. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  2. Other Protocols: Nuclei Chromatin Extraction, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: S2-Mt-Bio-H2Av, S2-Mt-Bio-H3.3, S2-Mt-Bio-H3, S2-Mt-Bio-H2A
  2. External Links: GSM333863, GSM333852, GSM333854, GSM333834, GSM333850, GSM333855, GSM333842, GSM333838, GSM333856, GSM333851, GSM333859, GSM333832, GSM333853, GSM333847, GSM333846, GSM333858, GSM333861


Release Date: 2009-05-03 (80-150 mM Salt Extracted Chromatin H2Av,80 mM Salt Extracted Chromatin H2A,80-150mM Salt Extracted Chromatin H3.3,80-600 mM Salt Extracted Chromatin H3.3,80-600 mM Salt Extracted Chromatin H2Av,150-600 mM Salt Extracted Chromatin H2Av,150-600 mM Salt Extracted Chromatin H3.3,150-600 mM Salt Extracted Chromatin S2,H3.3 80 mM Salt Extracted Chromatin) Release Date: 2009-11-30 (H3.3 350 mM Salt Extracted Chromatin,H3.3 600 mM Salt Extracted Chromatin)