Chromatin NaCl fractions (S2-DRSC cells)

Salt Extracted Chromatin S2 (Henikoff project,Henikoff subgroup)

General Description

Chromatin from Drosophila S2 tissue culture cells was digested with micrococcal nuclease and extracted with 80 mM NaCl. The soluble fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.

Protocols

  1. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  2. Other Protocols: Nuclei Chromatin Extraction, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: S2-Mt-Bio-H2Av, S2-DRSC
  2. External Links: GSM333843, GSM387600, GSM387594, GSM333866, GSM387595, GSM387593, GSM333839, GSM333836, GSM333867, GSM387598, GSM333864


Release Date: 2009-03-22 (600 mM Salt Extracted Chromatin Pellet,150 mM Salt Extracted Chromatin) Release Date: 2009-05-03 (80-150 mM Salt Extracted Chromatin S2,80 mM Salt Extracted Chromatin S2,150-600 mM Salt Extracted Chromatin S2) Release Date: 2009-11-30 (600 mM Embryonic Salt Extracted Chromatin Pellet)