NimbleGen Tiling Arrays

Drosophila RNA Expression Tiling Array (Henikoff project, Ahmad subgroup)

General Description

Drosophila melanogaster Kc167 cells were H2Av RNAi treated and collected for RNA expression tiling arrays. poly A+ RNA was isolated and used to synthesize single-stranded cDNA probes. Labeled cDNA and labeled genomic DNA were hybridized to NimbleGen microarrays (2-color hybridizations). The hybridization signal was compared between genomic DNA and cDNA.  Scaled log2(ratios) of signal intensity are shown.These experiments are used as controls for nucleosome salt fractionations.

Protocols

  1. Growth and isolation: Cell Culture, Sample Prep
  2. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  3. Other Protocols: RNAi in cell culture, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: Kc167, S2-DRSC


Release Date: 2010-05-14