Chromatin NaCl fractions (wing disc)

Fly wing disc Salt Extracted Chromatin (Henikoff project,Henikoff subgroup)

General Description

Chromatin from Drosophila D16 tissue culture cells (derived from wing imaginal discs) was digested with micrococcal nuclease and extracted with 150 mM NaCl. The soluble fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.

Protocols

  1. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  2. Other Protocols: Nuclei Chromatin Extraction, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: ML-DmD16-c3


Release Date: 2009-11-24 (Fly embryos 1-3 hr 600 mM Salt Extracted Chromatin,Fly embryos 4-6 hr 150 mM Salt Extracted Chromatin,Fly embryos 1-3 hr 600 mM Salt Extracted Chromatin Pellet,Fly embryos 1-3 hr 150 mM Salt Extracted Chromatin,Fly embryos 4-6 hr 600 mM Salt Extracted Chromatin,Fly embryos 4-6 hr 600 mM Salt Extracted Chromatin Pellet) Release Date: 2009-11-30 (600 mM Embryonic Salt Extracted Chromatin Pellet,600 mM Embryonic Salt Extracted Chromatin,H3.3 600 mM Salt Extracted Chromatin,80 mM Embryonic Salt Extracted Chromatin)