Histone Variants

Histone Variants (Henikoff project, Henikoff subgroup)

General Description

Chromatin from Drosophila S2 tissue culture cells engineered to express biotin-tagged histone H2A or H2Av was digested with micrococcal nuclease and extracted with 15 mM NaCl, sheared, and concentrated with streptavidin-coated beads. The bound fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.

Protocols

  1. Sample preparation: Strand-Displacement Labeling, NimbleGen Hybridization, NimbleGen Scanning
  2. Other Protocols: Nuclei Chromatin Extraction, NimbleGen Scaling

Experimental Reagents

  1. Arrays: 070928_Henikoff_Dmel_r52_ChIP

Sample Details

  1. Animals/Lines: S2 S2
  2. External Links: GSM333849, GSM387597, GSM391381, GSM333848, GSM387596, GSM391380


Release Date: 2009-03-22

Drosophila Core Histone H3 Binding Sites (Henikoff Lab). Chromatin was prepared from Drosophila S2 cells engineered to express histone H3 tagged in vivo with biotin. After digestion with micrococcal nuclease and collection with streptavidin-coated beads, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down by the streptavidin beads. Scaled log2(ratios) are shown in this track. [PMID: 17347439]
Drosophila Variant Histone H3.3 Binding Sites (Henikoff Lab). Chromatin was prepared from Drosophila S2 cells engineered to express a histone variant, H3.3, that was tagged in vivo with biotin. After digestion with micrococcal nuclease and collection with streptavidin-coated beads, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down by the streptavidin beads. Scaled log2(ratios) are shown in this track. [PMID: 17347439]