Histone VariantsHistone Variants (Henikoff project, Henikoff subgroup)General DescriptionChromatin from Drosophila S2 tissue culture cells engineered to express biotin-tagged histone H2A or H2Av was digested with micrococcal nuclease and extracted with 15 mM NaCl, sheared, and concentrated with streptavidin-coated beads. The bound fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.Protocols
Experimental Reagents
Sample Details
Release Date: 2009-03-22 Drosophila Core Histone H3 Binding Sites (Henikoff Lab). Chromatin was prepared from Drosophila S2 cells engineered to express histone H3 tagged in vivo with biotin. After digestion with micrococcal nuclease and collection with streptavidin-coated beads, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down by the streptavidin beads. Scaled log2(ratios) are shown in this track. [PMID: 17347439] Drosophila Variant Histone H3.3 Binding Sites (Henikoff Lab). Chromatin was prepared from Drosophila S2 cells engineered to express a histone variant, H3.3, that was tagged in vivo with biotin. After digestion with micrococcal nuclease and collection with streptavidin-coated beads, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down by the streptavidin beads. Scaled log2(ratios) are shown in this track. [PMID: 17347439] |