Chromatin Proteins in S2-DRSC cells

Chromatin-binding Proteins in S2-DRSC cells (Karpen project, Karpen subgroup)

General Description

We aim to determine the locations of 125 chromosomal proteins across
the Drosophila melanogaster genome. The proteins under study are
involved in basic chromosomal functions such as DNA replication, gene
expression, gene silencing, and inheritance. We will perform Chromatin
ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will
initially assay localizations using chromatin from three cell lines
and two embryonic stages, and will then extend the analysis of a
subset of proteins to four additional animal tissues/stages

Protocols

  1. Growth and isolation: Chromatin prep from cultured cells, Crosslinking of cultured cells, Crosslinking of cultured cells, Chromatin prep from cultured cells
  2. Sample preparation: Hybridization to Affy arrays, WGA, ChIP, Array Scanning Protocol
  3. Data Analysis: Regions of significant enrichment, Smoothed M-value enrichment profiles, M-value normalization, Regions of significant enrichment, Smoothed M-value enrichment profiles
  4. Other Protocols: Regions of significant enrichment, Regions of significant enrichment, Regions of significant enrichment

Experimental Reagents

  1. Antibodies: MBD-R2_Q2567, Ab:PR-Set7_Q3484:GK:1, SU(HW)-HB, Chro(Chriz)BR, HP1a wa184, Ab:MLE_Q4142:GK:1, WDS_Q2691, Su(Hw)-VC, RNA pol II (ALG), HP2 (Ab2-90), Ab:HP1b_Q4114:GK:1, Ab:MBD-R2(Q4167):GK:1, Chro(Chriz)WR, HP1c (MO 462), HP1b (Henikoff)
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R

Sample Details

  1. Animals/Lines: S2-DRSC


Release Date: 2010-09-27