Dosage comp. Proteins in Kc167 cells

Dosage compensation proteins in Kc167 cells (Karpen project, Kuroda subgroup)

General Description

We aim to determine the locations of 125 chromosomal proteins across
the Drosophila melanogaster genome. The proteins under study are
involved in basic chromosomal functions such as DNA replication, gene
expression, gene silencing, and inheritance. We will perform Chromatin
ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will
initially assay localizations using chromatin from three cell lines
and two embryonic stages, and will then extend the analysis of a
subset of proteins to four additional animal tissues/stages

Protocols

  1. Growth and isolation: Chromatin prep from cultured cells, Crosslinking of cultured cells
  2. Sample preparation: Hybridization to Affy arrays, WGA, ChIP, Array Scanning Protocol
  3. Data Analysis: M-value normalization, Smoothed M-value enrichment profiles
  4. Other Protocols: Regions of significant enrichment

Experimental Reagents

  1. Antibodies: Ab:JIL1_Q3433:GK:1, Ab:MOF_Q4145:GK:1
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R

Sample Details

  1. Animals/Lines: Kc167


Release Date: 2010-09-27