ChIP-Seq: Histone Modifications in L3 (H1, Karpen)

H1.D.mel 3rd Instar Larvae Nuclei.Solexa (Karpen project, Elgin subgroup)

Details

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General Description

We aim to determine the locations of the major histone modifications
across the Drosophila melanogaster genome. The modifications under
study are involved in basic chromosomal functions such as DNA
replication, gene expression, gene silencing, and inheritance. We will
perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina
NGS sequencing platform. We will initially assay localizations using chromatin from
three cell lines and two embryonic stages, and will then extend the
analysis of a subset of proteins to four additional animal tissues/stages.

Protocols

  1. Growth and isolation: Cage population, Larvae collection, Chromatin prep from tissues
  2. Sample preparation: ChIP, Illumina sequencing
  3. Data Analysis: Bowtie alignment
  4. Other Protocols: SPP Peak calling

Experimental Reagents

  1. Antibodies: H1

Sample Details

  1. Animals/Lines: Drosophila melanogaster


Release Date: 2013-03-17