H3 Histone Modifications in BG3 cells
H3K36me2_W.BG3.Solexa (Karpen project, Kuroda subgroup)Nuclei were isolated from the Drosophila BG3 neuronal cell line that had been crosslinked with formaldehyde. After lysis and sonication the chromatin was immunoprecipitated with an affinity-purified antibody that recognizes histone H3 dimethylated on lysine 36. The resulting fragments were used to create libraries and sequenced on the Illumina platform. Short reads were aligned to the reference genome using Bowtie, and peaks for each replicate were called using SPP (using sequence from the Input material as a comparison control). Statistically significant regions of enrichment from the two biological replicates were then combined and used to call the cumulative peaks.
We aim to determine the locations of the major histone modifications
Release Date: 2013-03-19