Chromatin Proteins in Kc167 cells
RLPII_phospho5_ab5131.Kc.Solexa (Karpen project, Kuroda subgroup)Nuclei were isolated from the Drosophila Kc167 embryonic cell line that had been crosslinked with formaldehyde. After lysis and sonication the chromatin was immunoprecipitated with an affinity-purified antibody that recognizes the large subunit of RNA polymerase II phosphorylated on heptapeptide serine 5 of its CTD. The resulting fragments were used to create libraries and sequenced on the Illumina platform. Short reads were aligned to the reference genome using Bowtie, and peaks for each replicate were called using SPP (using sequence from the Input material as a comparison control). Statistically significant regions of enrichment from the two biological replicates were then combined and used to call the cumulative peaks.
We aim to determine the locations of 125 chromosomal proteins across
Release Date: 2013-03-19 Submission 5571 Release Date: 2013-03-19 Submission 5571