Histone Modifying Enzymes in S2-DRSC cells

Histone Modifying Enzymes in S2-DRSC cells (Karpen project, Elgin subgroup)

General Description

We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages.

Protocols

  1. Growth and isolation: Chromatin prep from cultured cells, Crosslinking of cultured cells, Crosslinking of cultured cells, Chromatin prep from cultured cells
  2. Sample preparation: Hybridization to Affy arrays, WGA, ChIP, Array Scanning Protocol
  3. Data Analysis: Regions of significant enrichment, Smoothed M-value enrichment profiles, M-value normalization, Regions of significant enrichment, Smoothed M-value enrichment profiles

Experimental Reagents

  1. Antibodies: BRE1_Q2539, Su(var)3-7-Q3448, Ab:EZ-Q3421:GK:1, Ab:JHDM1_Q2634:GK:1, Ab:ASH1_Q4177:GK:1, Su(var)3-9, Ab:MRG15_Q2481:GK:1, NURF301_Q2602, Ab:Su(var)3-9-Q2598:GK:1, Ez, Ab:RPD3-Q3451:GK:1, Trx-C, dMi-2_Q2626, dRING Q3200
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R

Sample Details

  1. Animals/Lines: S2-DRSC


  1. Other Protocols: Regions of significant enrichment, Regions of significant enrichment


Release Date: 2010-02-15