H3 Histone modifications in CME W1 cl.8+ cells

H3 Histone modifications in CME W1 cl.8+ cells (Karpen project, Kuroda subgroup)

General Description

We aim to determine the locations of the major histone modifications
across the Drosophila melanogaster genome. The modifications under
study are involved in basic chromosomal functions such as DNA
replication, gene expression, gene silencing, and inheritance. We will
perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling
arrays. We will initially assay localizations using chromatin from
three cell lines and two embryonic stages, and will then extend the
analysis of a subset of proteins to four additional animal tissues/stages.

Protocols

  1. Growth and isolation: Chromatin prep from cultured cells, Crosslinking of cultured cells
  2. Sample preparation: Hybridization to Affy arrays, WGA, ChIP, Array Scanning Protocol
  3. Data Analysis: Smoothed M-value enrichment profiles, M-value normalization, Regions of significant enrichment, Smoothed M-value enrichment profiles
  4. Other Protocols: Regions of significant enrichment, Regions of significant enrichment

Experimental Reagents

  1. Antibodies: H3K9ac, H3K9me3 (new lot), Ab:H3K36me1:GK:1, Ab:H3K9acS10P_(new_lot):GK:1, Ab:H3K36me3:GK:1, H3K18Ac (new lot), H3K9me2-Ab2 (new lot)
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R

Sample Details

  1. Animals/Lines: CME W1 Cl.8+


Release Date: 2010-09-27