ChIP-seq: Chromatin Proteins in L3

dSFMBT Q2642.D.mel 3rd Instar Larvae Nuclei.Solexa (Karpen project, Elgin subgroup)

Details

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General Description

We aim to determine the locations of 125 chromosomal proteins across
the Drosophila melanogaster genome. The proteins under study are
involved in basic chromosomal functions such as DNA replication, gene
expression, gene silencing, and inheritance. We will perform Chromatin
ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will
initially assay localizations using chromatin from three cell lines
and two embryonic stages, and will then extend the analysis of a
subset of proteins to four additional animal tissues/stages

Protocols

  1. Growth and isolation: Cage population, Larvae collection, Chromatin prep from tissues
  2. Sample preparation: ChIP, Illumina sequencing
  3. Data Analysis: Bowtie alignment
  4. Other Protocols: SPP Peak calling

Experimental Reagents

  1. Antibodies: dSFMBT

Sample Details

  1. Animals/Lines: Drosophila melanogaster


Release Date: 2013-03-17