H4 Histone Modifications in L3 Oregon strain
H4K20me1 (3rd lot).D.mel 3rd Instar Larvae Nuclei.Solexa (Karpen project, Elgin subgroup)Nuclei were isolated from nuclei isolated from Drosophila Oregon R L3 larvae that had been crosslinked with formaldehyde. After lysis and sonication the chromatin was immunoprecipitated with an affinity-purified antibody that recognizes histone H4 methylated on lysine 20. The resulting fragments were used to create libraries and sequenced on the Illumina platform. Short reads were aligned to the reference genome using Bowtie, and peaks for each replicate were called using SPP (using sequence from the Input material as a comparison control). Statistically significant regions of enrichment from the two biological replicates were then combined and used to call the cumulative peaks.
We aim to determine the locations of the major histone modifications
Release Date: 2013-03-12 Submission 4948 Release Date: 2013-03-12 Submission 4948