H4 Histone Modifications in Oregon strain
H4K16ac(M).14-16 hr OR embryo.Solexa (Karpen project, Karpen subgroup)Nuclei were isolated from staged Drosophila Oregon R embryos, 14-16 hr after egg laying that had been crosslinked with formaldehyde. After lysis and sonication the chromatin was immunoprecipitated with an affinity-purified antibody that recognizes histone H4 acetylated on lysine 16. The resulting fragments were used to create libraries and sequenced on the Illumina platform. Short reads were aligned to the reference genome using Bowtie, and peaks for each replicate were called using SPP (using sequence from the Input material as a comparison control). Statistically significant regions of enrichment from the two biological replicates were then combined and used to call the cumulative peaks.
We aim to determine the locations of the major histone modifications
Release Date: 2013-03-13 Submission 4938 Release Date: 2013-03-13 Submission 4938