L2 RNA-Seq Unique mapper
Dm Developmental Timecourse Expression RNA-seq L2 uniquely mapping reads (Celniker project, Graveley subgroup)
Unstranded libraries were created from polyA+ RNA isolated from staged L2 y cn bw sp D. melanogaster flies, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Unstranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)
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Release Date: 2012-03-20