CGH array scores (Tissues)

CGH array data (MacAlpine project, Orr-Weaver subgroup)

General Description

Most terminally differentiated Drosophila tissues are either polyploid or polytene. Unlike normal chromosomes, where the entire chromosome must be replicated exactly once, polytene chromosomes are often differentially replicated with many regions underreplicated and some overreplicated. We will characterize five different polytene tissues using comparative genomic hybridization (CGH) to identify differentially replicated regions of each chromosome. These studies will also identify tissue specific amplicons, where the replication mediated amplification of specific loci is essential for up-regulation of mRNA levels encoding proteins critical for development. The differential replication of polytene chromosomes in Drosophila will provide a unique opportunity to understand how developmental cues and chromosomal domains influence replication initiation.

Protocols

  1. Growth and isolation: Follicle_cell_collection:DM:1, Salivary_gland_collection:DM:1, DNA_isolation_WI:DM:1
  2. Sample preparation: Labeling_WI:DM:1, Agilent_hybridization:DM:1, Array_scanning:DM:1
  3. Other Protocols: Normalization_and_peak_calling:DM:1

Reagents

  1. Animals: Oregon-R_Orr-Weaver, Oregon-R, Y_cn_bw_sp, Drosophila melanogaster
  2. Arrays: Agilent_Dm_4.3_616_tiling Agilent_Dm_5.1_470_tiling
Release Date: 2009-02-13