ChIP-Seq dORC2 (Tissues)

dORC2 ChIP-Seq experiments (MacAlpine project, MacAlpine subgroup)

General Description

We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues.

Protocols

  1. Growth and isolation: Tissue collection:DM:1, Larvae_Collection:DM:1, Embryo collection generic:DM:1, ChIP_DNA_Library:DM:1, ChIP crosslinking and isolation from tissue:DM:1
  2. Sample preparation: ChIP:DM:1
  3. Other Protocols: Illumina_sequencing:DM:1, Illumina_seq_maq_processing:DM:1, ChIPseq_analysis:DM:1, ORC_ChIPseq_analysis:DM:1

Experimental Reagents

  1. Antibodies: Ab:dORC2:DM:1, Ab:RNA_Poll_II_(Upstate_05-623):DM:1

Sample Details

  1. Animals/Lines: SuUR, L3 stage wandering larvae, Oregon-R-modENCODE

Release Date: 2010-10-27 Overnight embryo PolII Release Date: 2010-10-28 Overnight embryo dORC2 Release Date: 2009-11-08 Salivary Gland dORC2 (Terry Orr-Weaver lab)