ChIP-Seq MCM

MCM ChIP-Seq experiment (MacAlpine project, MacAlpine subgroup)

General Description

We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues.

Protocols

  1. Growth and isolation: ChIP_crosslinking_and_isolation:DM:1, Cell_Growth_ChIP_Large_Scale:DM:1
  2. Sample preparation: ChIP:DM:1, ChIP_DNA_Library:DM:1
  3. Data Analysis: ORC_ChIPseq_analysis:DM:1, Illumina_seq_maq_processing:DM:1

Experimental Reagents

  1. Antibodies: Pan MCM2-7 Antibody

Sample Details

    Animals/Lines:

Release Date: 2010-02-02