OSS, Unique mapper

D.melanogaster Cell Line Expression RNA-seq OSS uniquely mapping reads (Celniker project, Graveley subgroup)

Details

Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster cell line Cell Lines OSS RNA-seq uniquely mapped reads, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 55437888
Mapped read count: 35426513
Alignment rate: 63%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Reads from unstranded-libraries were aligned to unique locations in the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011).

Protocols

  1. Data Analysis: Stranded paired-end alignment
  1. External Links: GEO:TMPID:SRR500470, SRR500470

Related modENCODE submissions:


Release Date: 2012-05-30