OSS, Unique mapper
D.melanogaster Cell Line Expression RNA-seq OSS uniquely mapping reads (Celniker project, Graveley subgroup)
Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster cell line Cell Lines OSS RNA-seq uniquely mapped reads, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
RNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Reads from unstranded-libraries were aligned to unique locations in the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011).
Related modENCODE submissions:
Release Date: 2012-05-30