Post-embryonic (Total)

Post-embryonic Signal Intensities (Celniker project, Gingeras subgroup)

General Description

We will generate over 600 RNA samples in biological triplicate and use them to generate expression profile maps detailing the sites of transcription across the fly genome using whole genome tiling arrays at 38 bp resolution as a broad survey of the transcriptome and 7 bp arrays resolution to identify at high resolution transcripts ends, splice sites, and small RNAs.

Protocols

  1. Growth and isolation: Population Cages, Animal Collection, RNA_extraction
  2. Sample preparation: CDNA_synthesis_for_Expression_Arrays, Labeling_of_cDNA_for_Expression_Arrays, Array_Hybridization_for_Expression_Arrays, Array_Scanning_for_Expression_Arrays
  3. Data Analysis: Data_Normalization_for_Expression_Arrays, Signal Graph generation, Transfrag Analysis

Reagents

  1. Animals: DevStage: L1 stage larvae, Drosophila melanogaster, Y_cn_bw_sp, L2 stage larvae, Drosophila melanogaster, Y_cn_bw_sp, White prepupae (WPP), Drosophila melanogaster, Y_cn_bw_sp, Adult female eclosion+1 day, Drosophila melanogaster, Y_cn_bw_sp, Affymetrix Drosophila Tiling Arrays v2.0R, Adult male eclosion+1 day, Drosophila melanogaster, Y_cn_bw_sp, Adult male eclosion+5 days, Drosophila melanogaster, Y_cn_bw_sp, DevStage: L3 stage larvae, Drosophila melanogaster, Y_cn_bw_sp DevStage:Pupae%2C_WPP_%2B_2_days:SC:1, Drosophila melanogaster, Y_cn_bw_sp, DevStage:Pupae%2C_WPP_%2B_3_days:SC:1, Drosophila melanogaster, Y_cn_bw_sp, DevStage:Pupae%2C_WPP_%2B_4_days:SC:1, Drosophila melanogaster, Y_cn_bw_sp, DevStage:WPP_%2B_12_hr:SC:1, Drosophila melanogaster, Y_cn_bw_sp, DevStage:WPP_%2B_24_hr:SC:1, Drosophila melanogaster, Y_cn_bw_sp, 12 h post-molt, Drosophila melanogaster, y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1], clear gut PS(7-9), Drosophila melanogaster, y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1], light blue gut PS(3-6), Drosophila melanogaster, y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]
  2. Arrays: Affymetrix Drosophila Tiling Arrays v2.0R
  3. Samples: Celniker/RNA:284, Celniker/RNA:286, Celniker/RNA:288, Celniker/RNA:290b, Celniker/RNA:292, Celniker/RNA:294, Celniker/RNA:326, Celniker/RNA:328, Celniker/RNA:330, Celniker/RNA:374, Celniker/RNA:376, Celniker/RNA:378, Celniker/RNA:386, Celniker/RNA:388, Celniker/RNA:390, Celniker/RNA:368, Celniker/RNA:370, Celniker/RNA:372, Celniker/RNA:380, Celniker/RNA:382, Celniker/RNA:384, Celniker/RNA:440, Celniker/RNA:442, Celniker/RNA:444, Celniker/RNA:344, Celniker/RNA:346, Celniker/RNA:348, Celniker/RNA:350, Celniker/RNA:352, Celniker/RNA:354, Celniker/RNA:356, Celniker/RNA:358, Celniker/RNA:360, Celniker/RNA:332, Celniker/RNA:334, Celniker/RNA:336, Celniker/RNA:338, Celniker/RNA:340, Celniker/RNA:342, Celniker/RNA:296, Celniker/RNA:298, Celniker/RNA:300, Celniker/RNA:452, Celniker/RNA:454, Celniker/RNA:456, Celniker/RNA:444, Celniker/RNA:446, Celniker/RNA:450
Release Date: 2009-01-28