Rotenone 8ug/ml L3

Dm Treatment Expression RNA-seq Rotenone Treatment_8ug/ml Third Instar Larvae multiply mapping reads (Celniker project, Celniker subgroup)

Details

Reads sequenced from stranded RNA libraries made from Third Instar Larvae Oregon-R Drosophila melanogaster treated with Rotenone Treatment 8ug ml were aligned to the D. melanogaster r5 genome using TopHat (allowing multiple genomic alignments with up to two mismatches).
Total read count: 144724278
Mapped read count: 137020987
Alignment rate: 94%

General Description

Stranded RNA-seq analysis was performed on poly(A)+ RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster genome using TopHat.

Protocols

  1. Data Analysis: TopHat stranded RNAseq alignment
  1. External Links: SRR103734, SRR103735

Related modENCODE submissions:


Release Date: 2012-02-07