S2R+, Unique mapper

D.melanogaster Cell Line Expression RNA-seq S2-R+ uniquely mapping reads (Celniker project, Graveley subgroup)

Details

Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster cell line S2-R+, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 82155673
Mapped read count: 54683651
Alignment rate: 66%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Reads were aligned to unique locations in the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011).

Protocols

  1. Data Analysis: Stranded paired-end alignment
  1. External Links: GEO:TMPID:SRR070266, GEO:TMPID:SRR070279, GEO:TMPID:SRR124149, SRR070266, SRR070279, SRR124149

Related modENCODE submissions:


Release Date: 2012-03-19