S3, Multi mapper

Dm Cell Line Expression RNA-seq S3 multiply mapping reads (Celniker project, Celniker subgroup)

Details

Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster S3 cell line, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned with TopHat against the r5 genome. Tracks show read alignments and density.
Total read count: 131644461
Mapped read count: 122745575
Alignment rate: 93%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and strand-specific library construction were performed in the Brenton Graveley lab. Libraries were distributed among 3 labs in the Drosophila Transcriptome group for sequencing (Celniker, Gingeras, and Graveley) for paired-end RNA sequencing (2x76+ nt) on the GAIIx platform. Fastq files were generated using pipeline version 1.5.

Protocols

  1. Data Analysis: TopHat stranded RNAseq alignment
  1. External Links: SRR189834, SRR070259, SRR189835, SRR111872

Related modENCODE submissions:


Release Date: 2012-05-22