S3, Multi mapper
Dm Cell Line Expression RNA-seq S3 multiply mapping reads (Celniker project, Celniker subgroup)
Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster S3 cell line, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned with TopHat against the r5 genome. Tracks show read alignments and density.
RNA-seq analysis was performed on poly(A)+ RNA from various cell lines of Drosophila melanogaster. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and strand-specific library construction were performed in the Brenton Graveley lab. Libraries were distributed among 3 labs in the Drosophila Transcriptome group for sequencing (Celniker, Gingeras, and Graveley) for paired-end RNA sequencing (2x76+ nt) on the GAIIx platform. Fastq files were generated using pipeline version 1.5.
Related modENCODE submissions:
Release Date: 2012-05-22