Agilent Array

Agilent Array experiments (White project, White subgroup)

General Description

The White Lab is aiming to map the association of all the Transcription Factors (TF) and DNA associated proteins on the genome of Drosophila melanogaster. The main technique that will be used for this purpose is chromatin immunoprecipitation-on-chip (ChIP-on-chip) utilizing whole-genome tiling arrays. The data generated by ChIP-chip experiments consist basically of a plot of signal intensity across the genome. The highest signals correspond to positions in the genome occupied by the tested TF.

Protocols

  1. Growth and isolation: Embryonic collection Stage 8-16 hours AEL:KW:1, Embryonic collection 1-6 hours AEL:KW:1
  2. Sample preparation: Array Scanning, White_Lab/IP, Array Hybridization for ChIP-chip:KW:1, Whole_Genome_Amplification, Agilent 244K ChIP Labeling:KW:1
  3. Data Analysis: Agilent ChIP data processing:KW:1, Agilent ChIP peak finding:KW:1
  4. Other Protocols: Cross-linking

Experimental Reagents

  1. Antibodies: No Antibody Control, KW4-E(z)-D2, odg-GFP, No Antibody Control, KW4-mod(mdg4)-D2
  2. Arrays: Agilent 1X1 M Drosophila Tiling

Sample Details

  1. Animals/Lines: Embryo 8-16h, Embryo 1-6h

Release Date: 2010-02-10