AdultMale

Track ID=YAdultMale_accepted_hits.sam.bam.sorted [Download entire dataset as BAM or BigWig]

AdultMale RNA-seq ( project, White subgroup)

General Description

RNA-seq was performed on polyA-RNA for developmental stages of Drosophila melanogaster on which ChIP-chip or ChIP-seq has been performed. The RNA was isolated from the same material used for ChIP experiments and, after purification and production of the Solexa libraries, sequenced (single-end) at Argonne National Lab. For RNA-seq, 2 lanes per sample are bieng run.

Protocols

  1. Growth and isolation: Animal Staging & collection, MRNA Extraction for Illumina Sequencing, RNA-seq Library Preparation for Illumina Sequencing
  2. Sample preparation: RNA-seq Library Preparation for Illumina Sequencing, Solexa_RNAseq
  3. Data Analysis: TopHat RNA-seq Alignments:KW:1

Sample Details

  1. Animals/Lines: AdultMale_TotalRNA, Adult_Male, y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]
  2. External Links: GSM451804

Release Date: 2009-10-04