Female Eclosion + 30 days RNA-Seq Multi mapper

Dm adult female, eclosion + 30 days RNA-Seq, multi mapper (Celniker project)


RNA-seq was performed on a single biological sample of D. melanogaster (y cn bw sp) adult females 30 days after eclosion, using a combination of single- and paired-end sequencing. Sequences were aligned to the FlyBase r5 genome using TopHat. Track displays alignment read density.
Uniquely mapped reads: 69534265
Multiply mapped reads: 654793
Total reads sequenced: 89411357

General Description

RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and library construction were performed in the Brenton Graveley lab. Libraries were distributed among 5 labs in the Drosophila Transcriptome group for sequencing. The Susan Celniker lab performed paired-end sequencing exclusively (2x76 nt) . The Brenton Graveley, Tom Gingeras and Michael Brent labs performed single-end sequencing exclusively (1x76 nt). The Brian Oliver lab performed both single- and paired-end sequencing. All labs used the same Illumina GAII platform and called bases using the Illumina processing pipeline. Fastq files were generated using pipeline version 1.3 before 5/07/09 and using version 1.4 after that date. Reads were aligned to the genome sequence using Tophat v.1.0.10.


  1. Growth and isolation: mRNA purification from total RNA
  2. Sample preparation: Sample prep
  3. Other Protocols: Paired-end Illumina Sequencing, Single-end Illumina Sequencing, TopHat alignment

Sample Details

  1. Samples: Celniker/RNA:400
  2. External Links: SRR023548 SRR023598 SRR023674 SRR023681, and 39 more.

Related modENCODE submissions:

Release Date: 2010-06-10