L3, wandering stage, fat body Unique mapper
Dm Tissue polyA Sites Expression RNA-seq third instar larvae wandering stage fat body uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)
Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster third instar larvae wandering stage fat body tissues, sequenced using Illumina GAIIx or HiSeq, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
RNA-seq analysis was performed on poly(A)-site enriched RNA from various tissues dissected from various stages of D. melanogaster. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011).
Related modENCODE submissions:
Release Date: 2012-04-25