L3, wandering stage, imaginal discs Unique mapper

Dm Tissue polyA Sites Expression RNA-seq third instar larvae wandering stage imaginal discs uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Stranded libraries were created from polyA+ RNA isolated from the D. melanogaster third instar larvae wandering stage imaginal discs tissues, sequenced using Illumina GAIIx or HiSeq, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 17426988
Mapped read count: 27485
Alignment rate: 0%
Only filtered reads are aligned, and therefore tallies reflect an artificially low mapping rate.

General Description

RNA-seq analysis was performed on poly(A)-site enriched RNA from various tissues dissected from various stages of D. melanogaster. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011).

Protocols

  1. Data Analysis: Stranded paired-end polyA-spanning Alignment
  1. External Links: GSM838752, GSM838786, SRR384911, SRR384945

Related modENCODE submissions:


Release Date: 2012-04-25