454 Mixed-stage Embryo (polyA+ RNA)
454 Sequencing of Drosophila melanogaster mixed stage embryos alignment (Celniker project, Celniker subgroup)
Unstranded libraries were created from polyA+ RNA isolated from the D. melanogaster mixed-stage embryos, and sequenced on Roche 454 platform. Reads were submitted to SRA.
RNA-seq analysis onthe 454 platform was performed on poly(A)+ RNA from selected developmental stages spanning the life cycle of D. melanogaster. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and library construction was performed by Blanchette, Cherbas, or Roche. Libraries were sequenced at Roche or in the Blanchette lab. SFF files were generated and submitted to SRA.
Referenced modENCODE submissions:
Release Date: 2012-08-25 Submission 5549 Release Date: 2012-08-25 Submission 5549