RSTs - CEUP1

Piano mixed-stage 3'RACE (Piano project, Piano subgroup)

General Description

The 3' untranslated region (3' UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3' UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

  1. Growth and isolation: RNA_extraction
  2. Sample preparation: UTRome RACE and cloning, Sequencing
  3. Other Protocols: RNA_extraction, Data Processing

Reagents

  1. Animals: Caenorhabditis elegans, Mixed Worm Stages, N2

Release Date: 2009-05-01