mRNA microarray N2 Embryos

C. elegans N2 Embryo mRNA Expression (Henikoff project)

General Description

C. elegans N2 Embryos were collected for mRNA expression tiling arrays. poly A+ RNA was isolated and used to synthesize single-stranded cDNA probes.
Labeled cDNA and labeled genomic DNA were hybridized to NimbleGen
microarrays (2-color hybridizations). The hybridization signal was compared between genomic DNA and cDNA.  Scaled log2(ratios) of signal intensity are shown.

Protocols

  1. Growth and isolation: Worm Growth and Harvest, Sample Prep
  2. Sample preparation: NimbleGen Hybridization, NimbleGen Scanning, Strand-Displacement Labeling
  3. Other Protocols: NimbleGen Scaling

Experimental Reagents

    Growth Conditions:
  1. Arrays: NimbleGen C elegans WS170 Tiling HX1

Sample Details

  1. Animals/Lines: Young Adult, N2


Release Date: 2010-02-20